Facts About Legionella

Assumed Legionella count in bulk water for a spa related to outbreaks is 1,000 to 3,600 CFU/ml Eurosurveillance Weekley May 20, 2004, 8(21)
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Heterotrophic bacterial CFU are often inversely proportional to numbers of Legionella in cooling tower samples, in our experience.
Routine biocide treatments will not eradicate Legionella bacteria in the environment, only in laboratory studies.
The superheat and flush method is not an effective method to eradicate legionella from water distribution systems (Acta Pathologica, Microbiologica et Immunologica Scandinavica113:45-53, 2005).
Culture methods are good during outbreaks for biotyping; but culture methods lack sensitivity for routine, quantitative monitoring. Many factors will inhibit growth or identification of legionella on BCYE with or without antimicrobial agents, heat or acid treatment.
Culture methods will not identify non-culturable legionella that can still cause outbreaks (non-culturable, viable legionella have been reported in several peer-reviewed journals, including Acta Pathologica, Microbiologica et Immunologica Scandinavica 113:45-53, 2005. The American Academy of Microbiology recommends new approaches for analyzing uncultivated microorganisms. Only DFA tests performed by trained laboratory personnel can identify these legionella.
Direct fluorescent antibody (DFA) tests using a battery of monoclonal antibodies provide more useful routine monitoring information than culture methods.
Legionnaires' Disease typically causes a serious pneumonia that requires prompt medical attention.
There are 8,000 to 18,000 cases of Legionnaires' Disease every year in the United States.
Prudent practice involves removal of Legionella bacteria that cause this disease from aerosol generating equipment when the bacteria multiply to high numbers.
Legionella species of bacteria cause Legionnaires' Disease. They are gram negative (but stain poorly), strictly aerobic rods.
Legionella infect people when they inhale aerosols (small particles that do not settle out of the air) containing these bacteria. Legionella dispersion can extend over a distance of at least 6 kilometers (Journal of Infectious Diseases 193:102-11, 2006)
Legionella are naturally found at the air-water interface in surface water, in ground water (Appl Environ Microbiol 71:663, Feb 2005), and in aerated biofilms.
Very low numbers of Legionella enter buildings in the water supply.
Legionella may multiply to high numbers in cooling towers, evaporative condensers, air washers, humidifiers, hot water heaters, spas, fountains, and plumbing fixtures.
Within one month, Legionella can multiply, in warm water-containing systems, from less than 10 per milliliter to over 1,000 per milliliter of water.
The highest number of Legionella that we have found in a water sample is 150,000 per milliliter of water.
Only 7% of the tower samples tested by our laboratory have Legionella counts greater than 200 per milliliter.
The U.S. Environmental Protection Agency and the U.S. Occupational Safety and Health Administration recommend routine maintenance of water-containing equipment. GTS recommends monthly testing for Legionella as part of a routine maintenance program.
There are no federal or state certification programs for laboratories that perform Legionella testing of environmental samples. Therefore, care must be taken when selecting a testing laboratory.
Once high numbers of Legionella have been found, a relatively simple procedure for disinfecting water systems with chlorine and detergent is available. This procedure is not part of a routine maintenance program because equipment may become corroded.
Regular monitoring with a battery of DFA monoclonal antibodies for several serogroups and species of Legionella morphologically intact bacteria provides a means for exercising 'reasonable care' to deter potential litigation.

GTS Legionella Laboratory
P.O. Box 7555
413-B East Diamond Ave.
Gaithersburg MD 20898
(888) 948-4811 (Toll Free)
(301) 948-4121 (Phone)
(301) 948-6972 (Fax)

 

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