BMBL 6th Edition – Appendix A – Primary Containment for Biohazards – Revised June 2020 – Published November 17, 2020 – Order online before 6:30 AM EST at CDC INFO On-Demand – Publications.
Differs from current information in the –
NSF International Standard/American National Standard NSF/ANSI 49 – 2019 – Published March 1, 2020.
NSF 49 – 2019 Informative Annex 1 – Biosafety cabinet selection, installation, use, lifespan, and decommissioning –
This user manual is distributed for informative / educational purposes by NSF International.
This document is the United States informative reference for biosafety cabinets (BSCs).
BMBL 6 Information. NSF 49 Information.
Biological Safety Cabinets./ Biosafety Cabinets (NSF changed name many years ago).
Filter corrugated aluminum separators./ Corrugated aluminum separators not very common.
Canopy gap usually 1 inch. / Canopy gap varies.
Type C BSC hard duct exhaust outside./ Class II Type C1 BSC is exhausted to the outside via a canopy connection. [C1 BSC is similar to an A2 BSC].
NSF/ANSI Standard 49-2018./ NSF/ANSI Standard 49-2019 published on March 1, 2020.
Class II BSC 70% downflow. / Class II BSC downflow varies > 50% to 80%.
B2 BSC exhausts 1200 CFM room air. / B2 BSCs exhaust room air at a rate of Typically 175 CFM/ft (16.3 m3/m) of BSC width.
Laminar Flow “Clean Bench”. / Unidirectional Flow Clean-Air Device (IEST RP CC002) is the correct term.
BSCs are designed for 24-hour operation. / Non-B type BSC blower should be turned off and sash closed when operations are completed.
70% ethanol (EtOH) wiping. / 70% ethanol is not recommended as a work surface disinfectant.
1:100 dilution of bleach disinfection ./ Bleach corrodes stainless steel. Alternative disinfectants are recommended..
Touch-plate micro burners./ Electric furnaces. Flames should not be used.
BSC drain pan valve./ Drain valve removed on new BSCs.
Adequate clearance behind & sides./ 6” minimum clearance behind & on each side of BSC.
Table 2 [ I, B2 ]./ Table 2 [II, B2].
Figure 11 Vacuum Flask Diagram./ Liquid will shut off the vacuum when it reaches the sidearm – 2 flasks recommended with an airstone in the second flask.