Legionella Testing / Biosafety Training


Dr Richard W Gilpin’s Blog

Legionella Control Breaking News

Our laboratory has observed that water exposed to the environment contains numerous microorganisms that either inhibit legionella growth or overgrow legionella colonies on BCYE agar. This water also contains significant quantities of organic and inorganic materials that interfere with legionella molecular test methods. 

The National Academies of Sciences, Division on Earth and Life Studies will review the state of science with respect to Legionella contamination of water systems and issue a report that will:
1) describe the microbial ecology of water supplies;
2) identify primary sources and routes of human exposure to Legionella;
3) evaluate effective strategies for controlling and preventing Legionella;
4) review policies, codes, and guidelines that affect control and prevention of legionellosis; and 5) identify gaps in research and needs for additional research.

Comparison of culture, real-time PCR and next generation sequencing to detect legionella in cooling tower water. Samples were analyzed by a standard Legionella culture method, real-time polymerase chain reaction (RT-PCR), and 16S rRNA next-generation sequencing. All samples were negative by conventional Legionella culture. All water samples yielded positive results by real-time PCR (105 to 106 GU/L). 16S rRNA next generation sequencing showed high similarity and reproducibility among the water samples.